Dextran, Tetramethylrhodamine, 70,000 MW, Lysine Fixable

描述

Labeleddextransarehydrophilicpolysaccharidesmostcommonlyusedinmicroscopystudiestomonitorcelldivision,trackthemovementoflivecells,andtoreportthehydrodynamicpropertiesofthecytoplasmicmatrix.Thelabeleddextraniscommonlyintroducedintothecellsviamicroinjection.


DextranSpecifications:

? Label(Ex/Em):Tetramethylrhodamine(555/580)
? Size:70,000MW
? Charge:Anionic
? Fixable:FixableviaLysine

HighManufacturingStandardsofMolecularprobes?Dextrans
Weoffermorethan50fluorescentandbiotinylateddextranconjugatesinseveralmolecularweightranges.Dextransarehydrophilicpolysaccharidescharacterizedbytheirmoderate-to-highmolecularweight,goodwatersolubility,andlowtoxicity.Theyalsogenerallyexhibitlowimmunogeniticy.DextransareBIOLOGicallyinertduetotheiruncommonpoly-(α-D-1,6-glucose)linkages,whichrenderthemresistanttocleavagebymostendogenouscellularglycosidases.

Inmostcases,MolecularProbes?fluorescentdextransaremuchbrighterandhavehighernegativechargethandextransavailablefromothersources.FurThermore,weuserigorousmethodsforremovingasmuchunconjugateddyeaspractical,andthenassayourdextranconjugatesbythin-layerchromatographytohelpensuretheabsenceoflowmolecularweightcontaminants.

AWideSelectionofSubstituentsandMolecularWeights
MolecularProbes?dextransareconjugatedtobiotinorawidevarietyoffluorophores,includingsevenofourAlexaFluor?dyes(MolecularProbesdextranconjugates–Table14.4)andareavailableinthesenominalmolecularweights(MW):3,000;10,000;40,000;70,000;500,000;and2,000,000daltons.

DextranNetChargeandFixABIlity
Weemploysuccinimidylcouplingofourdyestothedextranmolecule,which,inmostcases,resultsinaneutraloranionicdextran.ThereactionusedtoproducetheRhodamineGreen?andAlexaFluor? 488dextransresultsinthefinalproductbeingneutral,anionic,orcationic.TheAlexaFluor?,CascadeBlue?,luciferyellow,fluorescein,andOregonGreen? dextransareintrinsicallyanionic,whereasmostofthedextranslabeledwiththezwitterionicrhodamineB,tetramethylrhodamine,andTexasRed? dyesareessentiallyneutral.Toproducemorehighlyanionicdextrans,wehavedevelopedaproprietaryprocedureforaddingnegativelychargedgroupstothedextrancarriers;theseproductsaredesignated"polyanionic"dextrans.

Someapplicationsrequirethatthedextrantracerbetreatedwithformaldehydeorglutaraldehydeforsubsequentanalysis.Fortheseapplications,weoffer"lysine-fixable"versionsofmostofourdextranconjugatesoffluorophoresorbiotin.Thesedextranshavecovalentlyboundlysineresiduesthatpermitdextrantracerstobeconjugatedtosurroundingbiomoleculesbyaldehyde-mediatedfixationforsubsequentdetectionbyimmunohistochemicalandultrastructuraltechniques.Wehavealsoshownthatallofour10,000MWAlexaFluor?dextranconjugatescanbefixedwithaldehyde-basedfixatives.

KeyApplicationsUsingLabeledDextrans
Thereareamultitudeofcitationsdescribingtheuseoflabeleddextrans.Someofthemostcommonusesinclude:

? Neuronaltracing(anterogradeandretrograde)inlivecells
? Celllineagetracinginlivecells
? Neuroanatomicaltracing
? Examiningintercellularcommunications(e.g.,ingapjunctions,duringwoundhealing,andduringembryonicdevelopment)
? Investigatingvascularpermeabilityandblood–brainbarrierintegrity
? Trackingendocytosis
? Monitoringacidification(somedextran–dyeconjugatesarepH-sensitive)
? Studyingthehydrodynamicpropertiesofthecytoplasmicmatrix

ForResearchUseOnly.Notintendedforanyanimalorhumantherapeuticordiagnosticuse.

ForResearchUseOnly.Notforuseindiagnosticprocedures.

規格

內容及儲存

Storeinfreezer(-5to-30°C)andprotectfromlight.

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